3’ Untranslated Regions (3’UTRs) influence gene function by controlling transcript stability, translational efficiency and mRNA transport. These functions are coordinated by 3’UTR interaction with specific cisinteracting molecules, including microRNAs and RNA-Binding Proteins (RBPs). The recent surge of highthroughput transcriptome sequencing has revealed a surprising diversity of 3’UTR isoforms. 3’UTR isoforms are regulated in a tissue-type and cell-type specific manner, and the longest 3’UTRs transcripts are expressed in the nervous system. To profile 3’UTR isoform diversity in Human DRG neurons, we performed Pacific Biosciences (PacBio) IsoSeq whole-transcript sequencing of RNA pooled from seven Human DRG samples. Limitations of the IsoSeq methodology are 1) it is cost-prohibitive for use with larger comparative experiments and 2) the resulting data is not quantitative across conditions. Therefore, we developed a pipeline employing an in-house developed algorithm - CSI-UTR - to analyze 3’UTR isoform expression in DRG neurons using standard mRNA libraries sequenced with Illumina technology.